SMF 2 Application Data Sheet No 7

Determination of proteins on surfaces

This datasheet describes the determination of Arginine, Ovalbumin and Human IgG on surfaces by a swabbing and fluorescence method, using a portable spectrofluorimeter.

Introduction

The SMF 2 instrument is designed to measure trace levels of fluorescent chemicals directly on surfaces for industrial hygiene, cleaning efficiency measurement and quality control. It is fully portable, produces a spectral output so that quantification is not solely dependant on single wavelength readings and has data logging capabilities.

It is anticipated that these methods will be of interest to companies operating biomanufacturing fermenters, those who process proteins in pharmaceutical plant and any organization which needs to measure and control these compounds on surfaces. 

Analytical methods

Water solutions of proteins and amino acids were pipetted directly onto filter paper for method development, or onto glass slides for simulation of a surface monitoring activity. Samples of 1-15 microgram of the protein were reacted with 15 microlitres of 1% triethanolamine and 15 microlitres of 0.2g/L Fluoram solution, either on the filter paper for method development or onto the paper wipe. Each yielded a fluorophor which was stable for several hours thus allowing efficient preparation of multiple samples and their measurement.

Readings were made after a drying and colour development period of 20 minutes had elapsed, with the sample held in the dark.